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EV: empty vector control; WT: non-optimized vector constructs; M1: epMEGAWHOP optimized vector constructs.
In all three cases an increased enzyme production was obtained with optimized vector backbones.
Constructs with an M1 label harbor an epMEGAWHOP optimized vector backbone.
These results prove that the lacI is an effective repressor even in the optimized vector backbone.
Through 36 consecutive cloning and 17 subcloning steps an optimized vector set was generated from the standard transfection plasmid.
We have shown that the implementation of optimized vector design, delivery regimens and appropriate delivery methods is critical to significantly increase epitope-specific antibody titers.
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Thus, an optimized vector-valued intensity measure, which includes the geometric mean of spectral accelerations over a range of period, is considered in the current study.
However, humanization is a must to overcome immunogenicity before considering for clinical use and optimized vectors for mammalian expression are also necessary for following industrialized manufacture.
The use of rAAV vectors expressing optimized vectors using MCK regulatory elements and, codon optimized cDNAs and hinge/repeat domain optimized micro-dystrophins has significant potential for clinical application in patients with DMD.
Utilizing recently developed low metabolic burden seed stock and fermentation strategies, optimized vectors can now be manufactured in high yields exceeding 2 g/L, with specific plasmid yields of 5% total dry cell weight.
Uninterrupted collection and processing of WNV-positive bird and mosquito pools since the 2002 introduction of WNV into Harris County has provided a conduit for the scientific investigation of real-time disease outbreaks with direct translation of findings towards optimized vector-borne disease control and prevention.
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