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The optimized time for growth was set at 5 days for optimizing the carbon and nitrogen sources as the melanin production is the same after day 5 and the conidial samples tend to become a dry mass of spores (Fig. 1).
As a results 150 s was observed as optimized time for the formation of ZnO NPs.
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The aliquot was shaken with optimized mass of different sorbents such as PSA, carbon black, and sawdust in batch cleaning and centrifuged for optimized time of 2 min at 4000 rpm.
The optimized time and temperature conditions for the LAMP assay were 60 min at 63 °C.
The compounds were cured for an optimized time interval on a hot press machine (Caver WMV50H, USA) maintained at 160 °C with applied pressure of ~11 MPa.
Cells were seeded at 5 × 10 mL–1 in a 96-well plate and treated with p53-MDM2 attagonists at various concentrations (0.1 50 μM) for the optimized time period of 44 h.
It is concluded from this experiment that there would be an optimized deposition time for clear pattern boundaries and high density of CNTs in the proposed method, and the excessive deposition of catalytic particles resulted in blurred boundary of CNT pattern and reduced density of the CNTs grown.
Many recent studies have investigated a suitable means for obtaining an "optimized" time-independent δ parameter.
The optimized shortest incubation time for nHAp synthesis was 3 h.
The optimized chamber saturation time for solvent system was 30 min at 25°C and relative humidity of 60%.
Therefore, 10 μmol/L and 5 d of induction were chosen as the optimized concentration and time for induction in the following experiment.
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