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In this paper, we optimized this method by running time evaluation using essential drugs defined by WHO and FDA-approved small-molecule drugs.
So, using the chemical conditions optimized, this method allows the determination of these elements employing the external calibration technique using aqueous standards with limits of detection and quantification of 0.04 and 0.14 µg L−1 for antimony, 0.04 and 0.14 µg L−1 for arsenic and 0.14 and 0.37 µg L−1 for selenium, respectively.
Based on value K that needs to be optimized, this method uses simulated annealing procedures to seek the best clustering option that can be defined between groups of populations among group genetic variation coefficients (F CT ).
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Optimizing this method's performance requires new measurement techniques compatible with the millimetre length scales and rapid separation dynamics observed in UTLC.
In order to optimize this method, the effect of the organic compounds and several components in the lysis buffer and the lysis and precipitation time were evaluated.
With the objective of optimizing this method, a 2 5-2) fractional factorial design was performed using data from UV vis spectroscopy analysis as a response surface.
The correct combination of these factors resulted in the enhanced invasion we were able to generate upon mechanical stimulation, however there are certain to be other factors that will further optimize this method.
Given the success of earlier efforts for the identification of novel viral nucleic acids using SISPA, we sought to adapt and optimize this method as a general and cost effective technique for large scale de novo viral genome sequencing.
To optimize this method, we used input genomic DNA from rice and arabidopsis, two model systems with well-characterized genomes [ 9, 10]. Figure 2 compares the effect of the order of size selection versus ligation to the adapters by comparing the yield and size of sequenced fragments to the total number predicted from the genome sequence of the target.
The objective function that is optimized in this method is the correlation coefficient (Formula 7).
The gene-specific primer pairs optimized for this method, the Tm as annealing temperature and the corresponding product size are summarized in Table 1.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com