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Using the cytotoxic potential as readout, we have optimized the procedure for oligomer preparation under cell-culture conditions with respect to Aβ concentration and time of incubation.
L.R.F. and S.B. prepped the animals, performed scans, and optimized the procedure.
HŠ and JB optimized the procedure for multiple displacement amplification of flow-sorted chromosomes.
In our methodology, we optimized the procedure and showed that during religation, going beyond certain minimum of DNA concentration favors self-circularization of individual fragments, which are poor candidates for MDA.
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To procure high quality and quantity of RNA after LCM, we optimized the procedures on tissue preparations and applied the approach for cell type-specific miRNA expression profiling in colorectal tumors.
An algorithm in three stages is built in order to optimize the procedure of discretization.
By optimizing the procedure we found the average level of carry-over after transfer of two pronuclei is less than 2.0%, with many of the embryos containing no detectable donor mtDNA.
It can be used to optimize the procedure of utilizing ammonia as a fuel in future equipment design.
As a method to deliver TFs into cells, we used synthetic mRNAs prepared by in vitro transcription (Supplementary Fig. S2a) based on our previous experience with mouse ESCs.21 After optimizing the procedure as described in the methods section, we used the method to transfect hESCs with synthetic mRNAs four times during the first two days (Supplementary Fig. S2b).
Moreover, several modifications were proposed to optimize the procedure of intersection analysis.
Many algorithms have been proposed to optimize the procedure for election of CH.
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