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We have optimized the method for use on an ABI 373 automated genotyping machine.
Stationary phase was selected on the basis of functional groups present in the compound; mobile phase composition was systemically studied and finally optimized the method conditions by using eco-friendly solvents.
Using the central composite design (CCD) approach, we optimized the method for analysis parameters including the electrode surface loadings of GO and AuNPs as well as the working solution pH.
Table 1 Tolerance of interference ions Interference Tolerance ratio in mass (w/w) (tested substances to analyte ratio) Zn2+ 200 Cu2+, tebuconazole 40 Triadimefon, 2-nitrophenol 20 Fe3+, NO3 −, SO4 2− 15 Carbendazim, benomyl, acetamiprid 15 4-Nitrophenol, phenol 15 Diuron 5. Once optimized, the method was finally characterized in terms of linearity, precision, accuracy, and sensitivity.
Natalie Rynkiewicz optimized the method for the lipid overlay assay.
TLF designed and optimized the method, performed mutation screening, and assisted in writing the paper.
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HT collected the urine samples, and optimized the methods used.
Alexei Brooun optimized the methods for PKN protein production.
Several ways are possible to optimize the method described here.
Experimental design was employed to optimize the method.
The labeling process is essentially the same from one compound to the next, requiring minimal efforts to optimize the method.
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