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As expression of GgPNO in E. coli also results in inclusion body formation, we have optimized the expression system.
We have optimized the expression of several far-red fluorescent reporters in M. tuberculosis by combining codon-biased synthetic genes with optimal promoters and replicons to produce reporter strains that can be detected with great sensitivity in vitro.
To reduce byproduct accumulation while maintaining xylose metabolism, we optimized the expression level of NADH oxidase by comparing its expression under the control of different promoters and plasmids.
When the various rare amino acid codons of apoptin were optimized, the expression level of the GST -TAT-Apoptinopt in E. coli BL21 DE3) was significantly further increased.
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A number of vectors are being designed to optimize the expression of transgenes.
A response surface method was adopted to optimize the expression conditions of the recombinant protein.
A series of modifications to optimize the expression of those enzymes and the medium have been done.
With additional background genome selection, the recurrent genome background can further improve the %RGC and optimize the expression of introgressed QTLs.
To minimize the possibility of selection of the undesired expression-improved mutants, the expression level of wildtype Rubisco was firstly maximized in E. coli through overexpressing the Rubisco-specific molecular chaperone and optimizing the expression conditions.
In the current report, we conducted a pilot antigen engineering study to optimize the expression and immunogenicity of subunit VP1 antigen for the design of EV71 vaccines.
The 3-AP titer was further increased to 3.94 g/L by optimizing the expression level of PPC using synthetic promoters and RBS sequences.
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