Sentence examples for optimized subsequently from inspiring English sources

The concentrations of three factors were optimized subsequently using central composite designs and response surface analysis.

These factors were optimized subsequently using Box Behnken design and response surface methodology.

The concentrations of the three factors were optimized subsequently using central composite design and response surface methodology.

Data generated in the process have been utilized for developing a fuzzy logic-based expert system, on the basis of the authors' knowledge, which has been optimized subsequently using a genetic algorithm (GA).

A colorimetric assay was used to pre-screen libraries for p-nitrophenol acetate hydrolysis activity and an HPLC-UV absorbance assay was optimized to subsequently screen for deacetylase activity toward hexa-N-acetyl-glucosamine substrate (GlcNAc 6.

Since PCR amplification of a 20-kb segment of the CBS gene proved to be unreliable, PCR conditions to amplify a shorter 12.2-kb portion of the CBS gene between exons 5 and 17 were optimized and subsequently employed.

18– 24 Our dengue PRNT uses the 50% neutralization potency estimate (PRNT50) and was optimized and subsequently validated according to the International Conference for Harmonization and Food and Drug Administration guidelines and shown to be suitable to evaluate the immunogenicity of dengue vaccine candidates.

The recording pipette was filled with Alexa-594 (10 µM in 2 M NaCl; Life Technologies, Carlsbad, CA) and visualized with an excitation wavelength of 800 nm, the minimum recording duration was 30 s. Images from eGFP and Alexa-594 were filtered using a Gaussian kernel, contrast-optimized and subsequently merged in Photoshop (Adobe, San Jose, CA).

A 24 full factorial central composite design was used to develop the quadratic model that was subsequently optimized and the optimal conditions were as follows: temperature 40 °C, pH 7.72, enzyme concentration 5.28 mg/g oil, buffer concentration 1 g/g oil and there was 65.5% conversion in 6 h.

In a previous study of five 17-amino acid peptides, we found little difference in relative energies between this procedure and another in which the side chains (in this case, the methyls) were subsequently optimized using DFT, with the (previously optimized) peptide chain held fixed.

60 The complexes were optimized in the gas phase and the optimized structures were subsequently subjected to a single-point energy calculation using the PBF solvation model 61,  62 with parameters suitable for NMP (dielectric constant, ε=32.2 and probe radius=2.67).