Additionally, a disruption and dilution sampling method, as described below as a previously optimized MSSA quantification standard protocol, was used as supplementary quantification methods to confirm the ability for the RODAC plates to recover cells from the surface after spray inoculation.
The TaqMan methodology was optimized for quantification of mouse GABA-A receptor subunits (α1 6, β1 3, γ2, and δ) and GAPDH.
To obtain accurate dosimetry, optimized activity quantification methods should be used, including methods used for scintigraphy (planar, SPECT), delineation of the organ, position of background ROI, attenuation and scatter correction, calibration, and late time points for imaging.
A solid phase extraction (SPE) method for extraction and clean up of 9 synthetic corticosteroids was optimized for quantification by reversed-phase high-performance liquid chromatography/negative electrospray ionisation mass spectrometry.
The photo-conversion hardware and geometry was optimized for quantification of subpopulations with extremely slow or no turnover, at the expense of introducing a multi-second dead time immediately following photo-conversion.
– A T2-weighted fast-recovery fast spin-echo sequence with fat suppression was optimized for quantification of the morphology (TR = 6,000 milliseconds; TE = 245 milliseconds; number of excitations = 2; echo-train length = 60; bandwidth = 20.83 Hz/pixel; field of view =16 cm × 16 cm; matrix = 320 × 320; nominal spatial resolution = 0.5 mm × 0.5 mm; slice thickness = 3 mm; slice gap = 0).
This study on prodromal AD patients recruited according to strict inclusion criteria revealed, using an optimized method of quantification on the cortex only, increased cortical amyloid load compared to cognitively normal subjects.
The optimized method allowed quantification of T6P in plant extracts with good linearity (r > 0.99) within a biologically relevant concentration range.
In contrast to targeted approaches that use optimized methods for the quantification of a set of predefined metabolites, non-targeted approaches usually provide less precise (relative) quantifications, but this for a biochemically broader range of metabolites.
These optimized reagents allow for quantification of the number of receptors expressed on the cell surface and a more accurate quantitation of receptor occupancy.
