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By twin-induced grain boundary engineering utilizing optimized one-step thermomechanical processing with 3% pre-strain and subsequent annealing at 1240 K for 72 h, a very high frequency of coincidence site lattice (CSL) boundaries (86%) was introduced into type 316 austenitic stainless steel.

All experiments described in this article use this optimized one-step PCR method (see Figure 2 for an example of agarose gel analysis of five different PCR segments).

The optimized real-time one step RT-PCR assay was compared with the diagnostic nested RT-PCR and different virus isolation systems [ in vivo (mice) in ovo (eggs), in vitro (Vero E6, PS, RD and Sand fly cell line)] for the detection of CHPV.

We report herein the synthesis of a series of positively charged potential penetration enhancers designed from d-glucose, 2-aminododecanoic acid, and additional lipophilic amino acids, and converted afterward into quaternary ammoniums in an optimized, innovative one-step reaction.

The entire procedure for training the proposed HDLA model is summarized in Algorithm 3. Initially, the parameters of CRSM module which takes the BoVW representation of each training image as input are optimized using one-step contrastive divergence algorithm with the update rules specified in Eqs.

Real-time one step RT-PCR was optimized using in vitro transcribed (IVT RNAA.

The optimized targets of freshwater and utility are also obtained in one step.

Under the optimized condition at pH 8.0, recombinant HP-NAP can be obtained in one step with high purity and high yield by collecting the unbound fraction from batch chromatography using DEAE Sephadex anion-exchange resin.

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