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Fig. 4 Difference assessment of cyanide degradation in basal medium (basic) and optimized medium (optimize).
An experiment was performed under the predicted optimal conditions in order to validate the optimized medium.
A verification experiment using the optimized medium was carried out.
Benchtop bioreactor tests with the optimized medium were performed.
In the optimized medium, bioluminescence per cell increased twofold.
Fermentations were carried out with the final version of the optimized medium (Table 1: medium C).
Daldinia eschscholzii fermentation process was performed in 500-L bioreactor based on the optimized medium.
Cultivation of isolate 5S in the optimized medium elevated biosurfactant production to 42.68% (according to emulsification index) from 31.74%, obtained in un-optimized medium.
Batch kinetics was also established in shake flask and fermenter using optimized medium and environmental conditions.
The data revealed that, optimized medium produces a higher concentration of enzyme than unoptimized medium.
In addition, the time course of elastase production in the optimized medium composition was also described.
