Glucose, olive oil and pH were essential to obtain a high lipase yield.
Thirty six percent of the soybean oil was converted into free fatty acids after hydrolyzing the oil for 12 h in the presence of optimized lipase.
Maximization of the lipase yield is a prerequisite for the immense potential of A. niger lipase.
Addition of soybean lecithin increased lipase production in 1.45-fold, presenting lipase yield (Y L / S ) of 10.061 U/g.
All cultures were developed in triplicate and the results were assayed for biomass, maximum lipase activity, biomass yield on substrate, lipase yield on substrate, biomass productivity, and specific rate of lipase production.
A high-resolution crystal structure of the optimized lipase provides insight into adaptations in PML leading to heat and MeOH tolerance.
The parameters, lipase yield (Y L / S = 10.061 U/g) and specific rate of lipase production (q L = 0.114 U of lipase/g of biomass·h), were considerably superior to the results observed without emulsifier (control).
However, in this study, the agitation speeds did not reduce period for production and increase lipase yield.
Other fermentation parameters analyzed in cultures performed at 210 rpm for 72 h showed biomass yield (Y X / S ) 1.456 g/g, lipase yield (Y L / S ) 6.638 U/g, biomass productivity (P X ) 0.303 g/h, and specific rate of lipase production (q L ) 0.062 U of lipase/g of biomass·h (Table 1).
Bench-top scale fermentation studies indicated that the recombinant carrying the codon-optimized lipase gene syn-lip under the control of promoter PAOX1 has a significantly higher lipase production capacity in the fermenter than other types of recombinants.
In these conditions, the lipase activity (LA) was predicted 343 U/mL that was approximately close to the predicted value (324 U/mL), which was increased 1.83 fold LA compared with the non-optimized lipase.
