In these conditions, the lipase activity (LA) was predicted 343 U/mL that was approximately close to the predicted value (324 U/mL), which was increased 1.83 fold LA compared with the non-optimized lipase.
Bench-top scale fermentation studies indicated that the recombinant carrying the codon-optimized lipase gene syn-lip under the control of promoter PAOX1 has a significantly higher lipase production capacity in the fermenter than other types of recombinants.
Using the Box-Behnken design, conditions were optimized for lipase production by Geotrichum candidum [ 34] and optimization of purified lipase from Cunninghamella verticillata for physical parameters was also shown [ 40].
A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate.
Forty-three percent of capsaicin (initial concentration of 500 mg/L) was converted to vanillylamine after treatment with the optimized medium (lipase activity 4559 U/ml) for 18 h.
Next, three most influential factors (Glucose, Urea and Olive oil) resulting from OFAT were optimized for lipase production by Trichoderma sp.1 using the central composite design under response surface methodology.
As many as 8 parameters such as the reaction medium, carboxylic acid type, carboxylic acid concentration, temperature, monoterpene type, monoterpene concentration, hydrogen peroxide concentration and amount of lipase were optimized using as less as 18 runs in triplicates (54 runs).
The composition of the optimized medium to enhance lipase production by D. hansenii is as follows (g/L): yeast extract 5.0, peptone 10, K2HPO4 4.0, Mglucose2O 13.1 glucolive3.1, oilve oil 19, Tween 80 3.8, and pH 6.4.
Under optimized conditions, the obtained lipase production showed good agreement between the experimental (49.37 U/g) and predicted (48.81 U/g) yields, with a coefficient of determination (R2) of 0.96.
