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Numerical multiple response optimization was applied to select an optimized formula (OF) with the goals of minimizing PS and maximizing ZP absolute value and EE.
Response surface methodology and multiple response optimization were used to search for an optimized formula.
The process was optimized for the dependent (response) variables Y1 to Y4, and the optimized formula was reached by keeping the bioadhesion force in target of 53.33 gm.
Optimized formulae were subjected to particle crystallinity and dissolution studies.
For the optimized formula, the loading efficiency was 42.50 wt %.
Lyophilization and sterilization did not affect the characteristics of the optimized formula.
The optimized formula was lyophilized, sterilized and imaged by scanning electron microscope.
Moreover, the in vivo behavior of the optimized formula was evaluated after IV injection in rabbits.
Further, the optimized formulae were incorporated in 1% Carbopol®-based gel.
The optimized formula was composed of 5 mg/mL span and 30 mg/mL polyvinyl alcohol.
The increase in relative bioavailability of TIZ from the optimized formula was 2.27 fold.
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