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The primers for nRT PCRs targeting icl2, hspx, and rRNAP1 genes were indigenously designed using the Primer-BLAST software, and optimized for sensitivity and specificity.
With the goal of developing a cell-based bioassay that is highly optimized for sensitivity, specificity, precision, and robustness, we performed 2 DOE experiments for assay optimization and 1 DOE experiment to validate assay robustness.
The running parameters were optimized for sensitivity and specificity.
For 50 bp Illumina data from a human genome, ten indexes and their associated masks were optimized for sensitivity (Table 1).
These methods are usually not optimized for sensitivity but for accuracy.
The exposure time was optimized for sensitivity and to achieve linearity.
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Accordingly, HPV typing of lesions in the present study was determined by PCR assay and hybridization/wash conditions were then optimized empirically for sensitivity and clean background using the appropriate HPV-type probe as indicated following the PCR.
The individual RT-PCR assays and the combined multiplex assay were optimized for highest sensitivity and specificity.
Both reactions were optimized for highest sensitivity and specificity and SG-based real-time was used to achieve quantitative detection.
The sensor is based on p-polarized reflectance, consisting of a sensing coated substrate, is easily optimized for maximum sensitivity in different applications.
Mathematical transformation technique is applied iteratively over the initial topology to generate a functional topology which is optimized for modulator sensitivity, hardware complexity and relative power consumption.
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