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Also, diagnostic procedures are not optimized for isolation of the different NTM species.
However, it is unlikely that every larval cell type can be successfully isolated and our protocol will need to be tested and optimized for isolation of specific cell lineages.
An efficient positive selection method has been optimized for isolation of SAM accumulating yeast strains with significant increases in SAM content on a dry cell weight basis.
Taken together, these data indicated that organic extraction protocols optimized for isolation of RNA also enrich for small DNA pieces.
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We describe optimized protocols for isolation of nuclear DNA from eight different plant species encompassing both monocot and eudicot species.
We describe optimized protocols for isolation of high quality, high molecular weight nuclear DNA from several species of plants with reduced levels of cpDNA and mtDNA contamination.
In this report, a semi-preparative HLPC and modified SALLE based methods were optimized for the simultaneous isolation and for the HLPC-DAD determination of khat alkaloids, respectively.
"I think what we bring to it is consistency that you know that every lot of the kit is going to look the same and the ease of use," says Yeh. Sigma-Aldrich currently offers over a dozen different organelle isolation kits, each optimized for a specific organelle or type of analysis.
Plant chromatin fixation and isolation protocols were optimized for developing xylem tissue collected from field-grown E. grandis trees.
We developed a technique for fixing in vivo fluorescence in brain cells and isolating them by LMD followed by an optimized RNA isolation procedure.
Libraries were size-selected by using a 2-step AMPure XP isolation method that was optimized for use in Ion 200 bp Template kits (16 ).
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