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Thermoresponsive polymer coatings, optimized for cell adhesion and thermally-triggered cell detachment, allow the fabrication of confluent cell sheets with intact extracellular matrix.
Multiple annulus repair strategies were developed using poly trimethylene carbonate) scaffolds optimized for cell delivery, polyurethane membranes designed to prevent herniation, and fibrin-genipin adhesive tuned to annulus fibrosus shear properties.
The array is a multiplex fluorescent sandwich ELISA that contains internal normalization, standards for quantification, and is optimized for cell culture supernatants.
Also, this assay was not optimized for cell lines, and cell lines were not readily available.
A therapeutic agent with a longer blood half-life that is also optimized for cell penetration could give substantially better results.
For example, our analysis of bacterial genomes being strongly optimized for cell growth made clear that aminoacyl-tRNA synthetases are overrepresented in the respective effectomes.
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Using a proposed model to describe the cell behaviour based on a 1-D approach, the contacted fraction of the rear contact was optimized for cells with and without BSF.
Once optimized for cell-based environments, the compound promises to disrupt N-linked glycosylation in a more refined fashion than tunicamycin.
In situ electroporation of monolayer cell cultures was performed as described before, according to a procedure that was optimized for cell-death studies.
However, many of these assays can be performed only on living cells and often are optimized for cells grown in tissue culture [ 20].
Thus cell culture conditions were optimized for each cell line in order to treat cells when RTCA cell index reached 0.5 1 for HepG2 and 0.5 1.5 for HuH7 cells one day after plating, that is, in linear phase of proliferation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com