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The system was quickly optimized (Figure 2), after individually testing 450 individual recipes from a space hundreds of times larger.
The only difference from the assay developed on anonymous leukopacks was the ratio between Tregs and responders, which we further optimized (Figure S1) due to limited number of available FACS-isolated Tregs from pediatric subjects.
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Lower cultivation temperature of 25°C under optimized conditions (Figure 5a and 5b), had effectively improved the bromelain production.
The m-ZrO2 sample was found to be the most effective catalyst in comparison with the t-ZrO2 and c-ZrO2 samples under identical optimized conditions (Figure 7).
The optimized fermentation (Figure 4) showed that best ethanol yield of 1.12 mol/mol-glycerol was produced after 48 h, although maximum productivity (0.74 g/l/h) was recorded after 24 h.
The adjusted proportional hazard ratio for prolonged mechanical ventilation was 2.6 times higher in CMV reactivating patients than in those who remained in a latent state (CI 95% 1.39 to 4.94, P < 0.001; optimized model; Figure 3).
The cells were washed to remove excess aptamer, and the aptamer was then cross-linked to the cells by incubating them for 2 min (time was optimized; Supplemental Figure 2, Supporting Information) in a 1% formaldehyde/PBS solution.
Finally, a permanent magnet with alternating high and low field regions is topology optimized and a Λcool figure of merit of 0.472 is reached, which is an increase of 100% compared to a previous optimized design.
Hence, 5.65-fold raise in overall bromelain production was achieved as compared to non-optimized conditions (Figure 2, in which maximum bromelain activity was 1.7 ± 0.03 U/mg).
The highest increase in light output achieved through addition of n-decanal was 58-fold in cells containing codon-optimized genes (Figure 4D), compared with only a 16-fold increase in light output from cell extracts co-transfected with the wild-type genes (Figure 4C).
TNT with open mouth-tube morphology was obtained after optimized ultrasonic agitation (Figure 2a). Figure 2b shows the bottom surface morphology of TNT after peeling-off from underlying Ti-substrate.
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