Sentence examples for optimized expression from inspiring English sources
Utilizing the optimized expression conditions, we expressed and analyzed both libraries by SDS PAGE gel electrophoresis and Western blotting.
Gene therapy is a logical approach, and recent work has focused on ways to optimize vector design and packaging to promote optimized expression and phenotypic rescue after intraocular delivery.
All mutant inhibitor proteins were expressed in E. coli (BL21DE3) using optimized expression conditions and purified by His-tag based affinity and size exclusion column chromatography.
The optimized expression system in this study was also applied for expressing another protein Ls-AChBP from Lymnaea stagnalis successfully.
As a proof of concept, we showed that the Arctic-sourced MZ0009 candidate, a putative GH18 family member, was expressed in a soluble and functional form in the optimized expression system.
The optimized expression for the two-dimensional flow velocity using MATLAB and R is expressed in Eq. 6.
To address the technical challenges of expressing Plasmodium proteins recombinantly, codon-optimized expression constructs containing an exogenous signal peptide and in which N-linked glycosylation sequons were mutated to prevent inappropriate glycosylation were expressed in mammalian cells (Crosnier et al., 2011; Bartholdson et al., 2012).
For codon-optimized expression of Drosophila FDL, a P. pastoris codon-optimized sequence was custom synthesized by GenScript, USA.
The superior bioluminescent production by cells containing pLuxCDEfrp CO/pLuxAB validates our dual plasmid, bicistronic, codon-optimized expression strategy and substantiates our hypothesis that the full bacterial lux cassette can be designed for functional autonomous expression in a mammalian cell line.
All codon-optimized expression constructs proved to lead to higher NTR levels as compared with the wild type cDNAs, using several independent batches of plasmid DNA.
To our knowledge, AGR2-targeted 18A4Hu I is a promising humanized anti-tumor drug candidate, and pHAb-FAST system is a useful optimized mammalian expression vector construction tool.
