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After 2 days, the J2s were separated from unhatched eggs and concentrated to a final optimized concentration of 1,000 J2 mL-1.
In the classical optimization, the 15% was found to be the optimized concentration, which agrees well with the previous studies, where 4 20% concentration was optimal (Houshmand et al. 2013; Kaur and Arora 2009; Sood et al. 2015).
Thus, β-CD at optimized concentration, i.e. 70 g/L was taken for oil optimization.
This is the reported optimized concentration of a TMB substrate [21].
This optimized concentration provides reasonable removal efficiencies and relatively low electrical energy consumption.
Artificial epidermal substitutes were then cryopreserved in trehalose at the optimized concentration.
Au silica NPs were introduced to the BHJ layer at an optimized concentration of 3 wt% with respect to P3HT.
The Colloidal gold conjugates were printed on conjugate pad at optimized concentration described later and dried at room temperature.
Thereafter, the reduced samples, CMDr and EMDr, were digested in a solution of sulfuric acid with optimized concentration and temperature.
The optimized concentration of the solvent was found to be 0.2 M in 30% isodecyl alcohol/n-dodecane.
Using the optimized concentration of critical elements, the biosurfactant yield showed close agreement with the model prediction.
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