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Beads from the optimized batches F9, F10, F11 and F12 were dispersed in the enteric coating solution and then stirred at 1200 rpm for 30 min at room temperature.
Similar findings have also been reported by Singh et al.. Percent drug loading and percent drug entrapment efficiency of the optimized batches were found to be 26.35±0.56 and 71.56±0.1.56, respectively (Table 3).
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Also, the optimized batch had stability over 6 months.
The final optimized batch was evaluated by various in vitro characterizations.
The optimized batch showed 100% Helicobacter pylori growth inhibition in 15 h in in-vitro culture.
Niosomal gel of optimized batch (F4) was prepared incorporating 2% w/w carbopol 934.
The optimized batch of formulation showed satisfactory yield (82.00 ± 1.18%) and drug entrapment efficiency (82.15 ± 1.35%).
The optimized batch had smaller globule size (<20 nm) and was stable for 3 months.
The optimized batch of TLM loaded nanosuspension exhibited a mean particle size of 334.67 ± 10.43 nm.
The developed optimized batch of microspheres ensured sustained release (>12 h) of ibuprofen.
Stability study of optimized batch shows no change in physical and chemical characteristics.
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