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Additionally, a fractional factorial design was used to shorten and optimize the sample extraction.
After choosing preliminary conditions, an experimental design was carried out with the variables C18, PSA, NaOH and CH3COONa to optimize the sample preparation step.
To optimize the sample preparation different precipitation methods have been tested.
In order to optimize the sample size, missing values were estimated using Expectation Maximization.
To optimize the sample concentrations and volumes for HiSeq sequencing we used a standard qPCR protocol for Illumina library preparations (KAPA library Quant Kit, Kapa Biosystems, Woburn, MA).
We used various incubation times for proteinase K treatment to optimize the sample pretreatment, measured in the quality of single-cell WGA DNA from cells exposed to CellSave by exome sequencing.
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A central composite design was used to optimize the sampling condition.
However, more investigation is required to improve the recovery performance and optimize the sampling and reconstruction processes.
This study presents a method to optimize the sampling design of environmental monitoring networks in a multi-objective setting.
The Austre Brøggerbreen and Edithbreen were considered as test sites to setup the experiment, to optimize the sampling strategy and to identify some basic experimental artefacts.
Due to the limited resources that are typically available for forest management, including the forest inventory phase, it is necessary to optimize the sampling approaches.
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