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A 5-level-3-factor experiment plan was used to optimize step deposition parameters.
Gaussian09 62) was used to optimize (Step 5) each conformer at the HF/6-31G** level of theory, with restraints on the ϕ and ψ angles to preserve the backbone secondary structure.
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In our approach, the combination of jointly optimized steps fuses several recently available techniques to a powerful cDNA-amplification and -cloning ensemble useful in high-throughput settings like those heading for protein production for structural biology or other demanding applications.
The method of combining theory with practice is used to analyze delamination of step drill, and to optimize the step drill structure.
To overcome these obstacles, Lipofectamine 3000 is designed to optimize every step in the transfection process and deliver superior performance and improved cell viability in hard-to-transfect cells researchers care about.
Response Surface Methodology (RS M and Central Composite Design (CCD) were used to model and optimize the step deposition parameters, such as initial potential, scan rate and peak potential.
The latter allows the modulation of the hydrophobic properties of the molecule to optimize the step of membrane protein extraction.
Since the analysis of plasma proteome necessarily requires a multidimensional approach, it is particularly important to optimize each step, in order to get the best results.
Our main work was to optimize each step.
The backtracking parameter λ can be used to optimize this step by choosing an appropriate value between zero and one.
Since no substantial efforts were made to optimize this step, we did not include it when measuring the overall computation time of b-MRP and t-MRP methods.
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