Sentence examples for optimize expression of from inspiring English sources

IRESs activated in ischemic conditions will be used in gene transfer IRES-based vectors to optimize expression of therapeutic genes in the gene therapy approach of heart ischemia developed in the laboratory.

In this study, we used the BglBrick standard cloning vectors to optimize expression of genes in a heterologous mevalonate-based isoprenoid biosynthetic pathway and examined their impact on production of amorpha-4,11-diene, an intermediate in the biosynthesis of the antimalarial drug artemisinin.

To optimize expression of the amiRNA, we chose to employ an endogenous rice miRNA precursor, a 245 bp fragment of the osa-MIR528 locus.

We hypothesize that HSF-1 and DAF-16 can interact within intestinal cells to optimize expression of HSF-1 and DAF-16 target genes that may extend lifespan.

However, in order to optimize expression of amiRs in M. truncatula, we decided to use an endogenous miRNA molecule as precursor.

Selection of a suitable temperature is also crucial to ensure that a balanced flux is maintained throughout the stages of protein synthesis (transcription) and maturation (translocation and periplasmic processing), in order to avoid accumulation of immature precursors and optimize expression of the pac gene [ 10].

Finally, by optimizing expression of hmaS, hmo and hpgT and attenuation of l-phenylalanine transamination, the l-Phg yield was increased by 224-fold comparing with the original strain.

Fine-tuned gene knockdown by controlling sRNA abundance will be useful for rapid design of microbial strains through simultaneously optimizing expression of multiple genes at a systems level, as it overcomes the difficulties of constructing and testing many different sRNAs and checking their cross-reactivity.

Here we optimized expression of the human wtPEDF gene using bacteria preferred codons according to the GeneOptimizer software algorithm (GeneScript, CA) for expression in the pET32a vector and presented the comparative sequence in Figure 1.

Overall, all of these relatively new approaches are based on spatial, temporal, and quantitative regulation of gene expression at the transcriptional or translational level, or both, thereby enabling production of higher amounts of specialized metabolites by overcoming bottlenecks, optimizing expression of genes, and redirecting the flux of precursors.

Clearly, there is still considerable room to optimize expression to maximize recovery of heritable mutations while simultaneously minimizing cytotoxic effects and off-target cleavage.