Sentence examples for optimize expression from inspiring English sources
To optimize expression, two codon-optimized coding regions have been designed and expressed alongside the wild-type coding region.
SDS PAGE analysis was carried out to check protein size and to check and optimize expression.
We therefore re-engineered the TFIIE subunit production vectors and tested various Escherichia coli host strains to optimize expression.
Further experiments were conducted using pFab3 to optimize expression levels using different strains of E. coli and various culture conditions.
The codons for the 2A and 1D regions were adapted to the codon usage of S. cerevisiae to optimize expression in yeasts (Fig. 1).
IRESs activated in ischemic conditions will be used in gene transfer IRES-based vectors to optimize expression of therapeutic genes in the gene therapy approach of heart ischemia developed in the laboratory.
Finally, by optimizing expression of hmaS, hmo and hpgT and attenuation of l-phenylalanine transamination, the l-Phg yield was increased by 224-fold comparing with the original strain.
Here we optimized expression of the human wtPEDF gene using bacteria preferred codons according to the GeneOptimizer software algorithm (GeneScript, CA) for expression in the pET32a vector and presented the comparative sequence in Figure 1.
By optimizing expression vectors and expression conditions, all BVMOs were obtained in a soluble form.
Optimizing expression is also important, and coregulating a xylose isomerase with downstream enzymes may prove effective.
As for construction of microbial cell factory, improving and optimizing expression levels of the enzymes in the metabolic pathway was always an important strategy; however, concentrations of specific enzymes could not be endlessly increased.
