Our first goal was to determine whether this artificial transcriptional cascade worked in a transient mode, and if so, to optimize the dynamic range of gene expression.
To optimize the dynamic range of the screen and to focus on early translational effects elicited by eIF2α phosphorylation, we incubated cells for 7 hr, at which time full induction of the reporter was reached.
To optimize the dynamic range of microarrays and allow the quantification of both high and low copy expressed genes, the same arrays were scanned using different photomultiplier settings allowing selection of images with saturated image intensities on the 50 and 100 μM EC spots and on oligonucleotide spots for high-abundance genes such as Rpl5.
In this paper, we describe our efforts to overcome the above limitations, focusing on increasing phosphorescence quantum yield, improving efficiency of the antenna-core energy transfer, minimizing quenching of phosphorescence by undesirable intramolecular pathways, and optimizing the signal dynamic range.
This results in a low positive number that can be tweaked from experiment to experiment to optimize the scale and dynamic range.
Integrated, collaborative efforts are needed to standardize a multi-modal set of biomarkers with dynamic range, optimize the methods, and conduct sufficiently powered, multi-site studies so that these tools progress rapidly to clinical qualification by regulatory agencies.
In addition, the linear k-space sampling used in this study is preferred to further increase the dynamic range and optimize the sequence for CA transit time.
These findings are very important to know when designing probes for this type of sensor in order to optimize the use of the dynamic range of the photodetector as discussed in further details in section 4. Figure 7(b) shows the collected photon fluence as a function of separation and penetration depth of each photon.
The design is optimized for low noise, high dynamic range, and low power dissipation.
