Exact(26)
Basic environment, to optimize culture conditions and after obtaining the optimal environment optimized by one way ONOVA vs. t test.
Response surface methodology, which allows for rapid identification of important factors and optimization of them to enhance enzyme production, was employed here to optimize culture conditions for the production of cis-epoxysuccinic acid hydrolase from Bordetella sp. strain 1 3.
This test might be used to predict damage to cells in processing equipment and to optimize culture conditions.
To optimize culture conditions, good understanding of these parameters is necessary.
To optimize culture conditions for human testicular somatic cells (TSCs) and spermatogonial stem cells.
Therefore, considerable effort has been ongoing in the research community to optimize culture conditions for predifferentiation of MSCs.
Similar(34)
After confirming the lack of pathogenicity (absence of ochratoxin A and fumonisin B2 production) this fungus was submitted to the experimental design in order to evaluate the effect of different variables on the enzymatic production, with the aim of optimizing culture conditions.
Optimizing culture conditions and developing high cell density culture techniques are imperative in future work [ 60].
10.7554/eLife.08413.017 Figure 4. Optimized culture conditions are required to generate and maintain MP cells.
Optimized culture conditions in flasks were also used for the 5 L fermentor with a working volume of 3 L.
Previous attempts to produce high yielding strains required selection of the genetic material through classical mutagenesis for commercial production of secondary metabolites, optimizing culture conditions, and random selection.
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