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Surprisingly, Tyr still dominates the YSX paratope and the additional amino acid types are primarily used to conformationally optimize contacts made by tyrosines.
Ligands identified in our study can be likely improved through further modifications to replace these water molecules and optimize contacts with the protein.
Small molecules that bind in the active site cleft typically induce a change in the relative positions of the small and large lobes, in part because the hinge that mediates domain closure typically forms direct interactions with compounds that occupy the adenine subsite and also because the two lobes can often adjust their relative orientation to optimize contacts with the bound inhibitor.
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We also tested the possibility to optimize contact formation by different implementations of double metal printing (DP) process.
In conjunction, a protocol to link the biological elements to the tips of carbon nanotubes has been developed to optimize contact and geometry between the redox enzyme and the carbon nanotube electrode array.
To optimize contact, electrodes and skin were wetted with water.
Centers aimed to optimize contact frequency such that attrition was prevented but participants were not overly bothered.
A water-soaked sponge was placed beneath the electrodes to optimize contact with the scalp, and the electrodes were held in place by adjustable rubber straps placed around the head.
The impedance of the electrode-skin interface was kept to a minimum by rubbing the skin with an EEG prepping gel, and conductive EEG paste was used to optimize contact with the electrodes.
The impedance of the electrode-skin interface was kept to a minimum by massaging the skin with an EEG prepping gel (Nu-Prep gel, DO Weaver & Co., CO, USA), and conductive EEG gel was used to optimize contact with the electrodes (Onestep-Clear gel, H+H Medical Devices, Dülmen, Germany).
The results can offer valuable insights for optimizing contact-free sensor designs.
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