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The translational coupling system was used to determine that a large multi-domain enzyme was not actively translated in E. coli, to isolate the translation problems to the C-terminal domains, and to optimize conditions for expressing a codon-optimized sequence variant.
A central composite design was used to optimize conditions for simultaneous extraction.
Additional humidifiers were added to counteract low humidity and helped optimize conditions for the sweet potato experiment.
In this study, response surface methodology was employed to optimize conditions for the extraction of barley β-glucan.
Effect of various parameters like pH, contact time, initial concentration and different electrolytes was investigated using batch process to optimize conditions for maximum adsorption.
The effect of pH, contact time, initial metal ion concentration and temperature on the adsorption was investigated using batch process to optimize conditions for maximum adsorption.
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We also optimized conditions for the extraction of intact native dodecameric Hfq complexes.
The statistically optimized conditions for feature extraction for all nine scoring algorithms in EvoGrader are shown in Table 2.
To develop La-targeted radioimmunotherapy, we optimized conditions for radioimmunoconjugation of the DAB4 clone of the La-specific mAb, which is represented by the APOMAB® trademark [22].
In the process of optimizing conditions for HIV-1 infection of mouse T cells, we found that additional TCR signals were essential, in a limited time window, for infection of pre-activated T cells.
In the limited time available for revision we were unfortunately unable to re-optimize conditions for this experiment, but we note that several reports (e.g. Star et al., 2002, Nat Neurosci; Sharma et al., 2006, Mol. Cell. Neurosci; Zheng et al., 2010, J. Neurosci) have already demonstrated very rapid FRAP for cytosolic GFP in dendritic spines (time constant <1 sec; Star et al., 2002).
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