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We show numerous optimizations of the protocol that make it efficient and practical.
Importantly, the optimizations of the protocol performed herein allow us to use 4×106 BM cells per cryovial, which give rise to about 10 million BMDM per cryovial.
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Previous optimization of the protocol showed typical purity was 99% or greater by a haematoxylin and eosin stain.
Since this protocol is still not applicable to a low VL range of HIV-1 infected plasma and serum specimens, further optimization of the protocol in the future will be necessary.
Once these two markers are fully established, an optimization of the protocol to a single-step protocol avoiding the expensive DNA sequencing and based on the detection of SNPs, such as PCR-RFLP, multiplex PCR, Real-time PCR, or other, can be realized.
After optimization of the protocol, a relatively pure protein fraction of TN122 was obtained).
Bryan Frank contributed significantly to the development, implementation, and optimization of the protocol.
In order to do the re-staining, we performed an optimization of the protocol.
Phylogenetic footprinting was applied at different stringency, to allow the following optimization of the protocol (below).
Thus, epigenomic profiling by ChIP-Seq will require further optimization of the protocol.
However, additional optimization of the protocol may be necessary before the technique can reliably estimate fly age.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com