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Those targeting systems which have shown promise in laboratory included chemical modification of the photosensitizer itself, drug delivery strategy optimization, the usage of antibodies and bacteriophage, and conjugation with traditional antibiotic [ 61– 61].
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This optimization technique is developed to optimize the usage of power.
The optimization was achieved via the usage of genetic algorithm.
The presenting strategy indeed provides an alternative way of handling the engineering optimization dynamically and expands the usage of ODE.
Details of this analysis can be found in Additional file 2. Earlier codon optimization studies have recommended the usage of high expression genes to design the recombinant gene for efficient heterologous expression [ 12, 13, 34].
The optimization of codon usage allows improving the translational efficiency of foreign proteins by replacing the codons which are rarely found in the host organism [ 28], and it has been introduced into many heterologous systems [ 29- 31].
Different modifications of the gene encoding PCV2 protein were also necessary for generation of recombinant PCV2 Cap protein including removal of the N terminal part with nuclear localization signal and fusing it with different tags or yeast signal peptide as well as the optimization of codon usage according to the host [ 16- 21].
Then, the difference between the proposed method and [12] lies in the usage of different optimization procedures for solving the H 2 norm objective function.
SIDe-O is a computer-aided design toolbox developed for the design of integrated inductors based on surrogate modeling techniques and the usage of evolutionary optimization algorithms.
Both algorithms are GA-based and they both consider several objectives regarding the optimization of resource usage and sharing at the substrate layer.
These include cultivation parameters, the effect of fusing the protein to an affinity tag and the optimization of codon usage of foreign gene for E. coli.
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