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After optimization, temperature and fermentation time, were set to 25.5 °C and 67.8 h.
This study involves three parts: (i) solvent optimization (ethanol and water), (ii) process optimization (temperature and time) for anthocyanin extraction and (iii) determination of stability of anthocyanins extracted under optimum conditions determined with respect to pH and temperature.
Following the optimization temperature (refer to result), the amplification of LAMP assay was performed at 58.5°C for 80 min and followed by 80°C for 2 min.
The other culture conditions were as fixed as follows, based on the results from the one-at-a-time optimization: temperature 30°C, shaking rate 80 rpm, inoculum 10% (v/v), cultivation time 30 h.
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This study utilizes finite element analysis for the optimization of temperature sensor The temperature sensor utilizes platinum RTD with a meander shape as a sensing element and parallel plate capacitive humidity sensor with array of hole at the upper electrode made of aluminum was design to operate in the range of -70°C to 70°C, 0% to 100% respectively.
Optimization includes temperature gradient selection of the annealing temperature, random population screening for common variants, and batch preparation of primer plates with robotically deposited and dried primer pairs.
The optimal SWE conditions obtained in simultaneous optimization were temperature of 200 °C, pressure of 30 bar and extraction time of 28.3 min, while obtained values of TP and TF yields and IC50 value at this experimental point would be 2.5452 g GAE/100 g CSS, 0.6311 g CE/100 g CSS and 0.01372 mg/ml, respectively.
In addition to the optimization of temperature, large cat/oil also facilitates the conversion.
Optimization of temperature and agitation speed for maximizing the FOS production was performed using response surface methodology.
Recent improvements of ATM transformation include the optimization of temperature and co-cultivation conditions, and the development of new selection markers (Michielse et al. [2005]).
The optimization of temperature profile has little influence on the production rate while the optimization of reactants pressure profile can significantly increase the production rate.
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