Literatures have highlighted the importance of optimization targets, restrictions and logistics design on the performance of bioenergy systems and regional bioenergy planning.
Multiresponse optimization with restrictions from practical considerations revealed that maximum saturated fatty acids composition in glycerides of the SL (38.6%) is obtained when SR = 3, E = 0.34 g and H = 3.2 ml are used.
The results of the new approach are compared to the results of the optimization without restriction on and the minimization of the number of feed streams.
The fingerprints obtained with primers 1846, 1854, and 1881 varied in individual amplified fragments, so the use of primers 1846, 1854, and 1881 for SSAP analysis of flax varieties will require further optimization of restriction, ligation, or PCR conditions.
This new protocol was simple and fast because it did not require labor-intensive optimizations in restriction enzyme digestion and ligation, did not involve special plasmid design, and enabled constructing a large-size mutant library for directed enzyme evolution within 1 day.
Topology optimization alleviates such restrictions and is capable of generating complex metallization patterns, which cannot be captured by the traditional optimization methods.
The use of Bayesian Networks allows to organize and correlate information gathered from different sources and its optimization may incorporate restrictions adjusting the network based on expert knowledge and network operativeness, in such a way that it may satisfactorily represent a given domain.
Taking advantage of the desirability concept and using the PoE measure as a response, a multiple optimization under different restrictions was carried out defining a new set of operating conditions able to target the maximum possible power density at the most robust conditions (1074 mW·cm−2 at 55 °C, 50% RHC and 25 Psi).
The DNA encoding S. pombe DUF2419 (abbreviated SpDuf2419, SPAC589.05c) was synthesized (without optimization) with added restrictions sites XhoI and BamHI at its 5′ and 3′ ends (GenScript USA Inc).
In principle, this method is generalizable to other genomes but may require optimization of the restriction enzymes used and fragment sizes amplified.
