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Therefore, a major challenge for the transfer of theoretical approaches to applications is the assessment and optimization of the methods' performance for supporting experimental research.
The findings of this study highlight the challenges involved in the diagnosis and control of FMD in endemic areas and emphasize the need for optimization of the methods used for serological diagnosis and for serotyping of FMDV outbreaks.
These results would suggest that hybridization conditions on the microarray are not stringent enough, however during initial optimization of the methods any increase in stringency resulted in a large loss of signal intensity (data not shown).
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These two compounds belonging to different chemical classes were chosen for the optimization of the method considering that the approach developed was optimized with respect to the radioligand binding assay.
A Response Surface Methodology was used for the optimization of the method.
After the optimization of the method, it was validated for its use for direct determination of carbonate in soil samples.
After optimization of the method, figures of merit were evaluated, as well as the ammonium concentration in some water samples.
The set-up is fully automated and features monitored extraction, which facilitates rapid optimization of the method.
It is expected that an even better sensitivity threshold could be achieved following further optimization of the method.
It is also shown, that a significant optimization of the method by reduction of a number of intensity values representing detected speckle patterns can be performed.
The study and optimization of the method was achieved through experimental design where recovery of compounds using acetonitrile for partition ranged from 62 to 99%.
More suggestions(16)
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streamline the methods
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