For optimum resolution in C3-corrected microscopes it is well established that a small negative value of C3, offset by positive values of C5 and defocus C1 results in the most optimal instrument resolution, and optimization of the CTF has been the subject of several studies.
Then we propose a convex optimization approach for learning the optimal instruments.
This result provides a quite general criterion for the optimization of instrument design.
Improvements in sample preparation and optimization of instrument parameters could increase the appearance of low abundant proteins in future studies.
In all cases, measurement sensitivity by fluorescence techniques requires standardization and optimization of instrument parameters [ 19, 23].
The McStas neutron ray-tracing simulation package is a versatile tool for producing accurate neutron simulations, extensively used for design and optimization of instruments, virtual experiments, data analysis and user training.
In matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), instrument optimization is a limitation restricting quantitation.
Here we describe optimization of various instrument and post-acquisition parameters that collectively provide for quantification of iTRAQ-labeled phosphorylated peptides isolated from complex cell lysates.
Most of the simulation tools the neutron scattering community has at their disposal target the instrument optimization until the sample position, with little focus on detectors.
While the optimization process is instrument-specific the principle is the same for all instruments, i.e., decrease the fragmentation while maintaining quasimolecular ion abundances.
