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This and several similar optimization experiments were performed in 96-well plate format.
Optimization experiments showed that a concentration of 20 mM of cPhe was best for V. cholerae counter-selection.
This prompted us to perform buffer optimization experiments, in order to identify buffers that reduce particle heterogeneity.
In the absence of such optimization, experiments have shown reasonable values are to set and to a value between 1.2 and 2 and.
We hence based the parameters employed in our optimization experiments on previously published reports which outlined stimulatory effects of intermittent loading for short durations at physiological frequencies, independent of the culture system used.
The optimization experiments could be simplified through a statistical design of experiments (response surface methodology).
Optimization experiments were carried out using a log transformed 5-level Box Behnken design.
· Optimization experiments without validation steps independent of the optimization runs.
All optimization experiments were performed without replication.
The optimization experiments were carried out in shake flask at 37 °C and 200 rpm.
This number is a result obtained from optimization experiments, not reported here.
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