In fact, murine TCR/human MHC-I-optimised interactions permitted us to study whether the murine CTL response was specific for four HLA-A02.01-restricted HLA-A02.01-restricted HLA-A02.01-restrictedman tumour cepitopess.
We tested if an optimised protocol to quantitate FGF8b protein expression could be developed to study archival prostate cancer specimens.
Using an optimised experimental approach, we developed Actichip, a custom oligonucleotide microarray designed to study the expression of actin cytoskeleton genes in various cell systems.
Fabrication parameters of this technique like annealing temperature and Eu3+ ion concentration have been studied and optimised to achieve maximum red emission from the Eu3+ ions.
The design of the current study was optimised to look at early latencies.
The concentrations of PMA and ionomycin used for in vitro stimulation in this study were optimised to ensure optimal cytokine production and minimal cell death.
The concentration of the VE1 antibody used in this study was optimised to maximise the sensitivity and specificity for BRAFV600E protein detection.
Our position is that the EEG may be insensitive to the frontal sources of the vMMN response, and that the MRI block design in the current study was optimised to detect frontal rather than occipital activation.
The PCR assays used in this study have been optimised to reduce to a minimum the rain effect, so that the setting of a threshold value to segregate positive droplets from negative droplets was not an issue.
Importantly, the sequencing techniques applied in these studies were not optimised to detect mutations that were only present in a small fraction of tumour cells within the sequenced samples.
