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Below, the main steps are discussed and the optimised steps in respect to the published literature are highlighted.
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Electrode design, optimisation steps and characteristics for the optimised electrode configuration are presented.
Whenever regression models are optimised, it is important that all optimisation steps are properly validated.
The results are compared to a non-optimised step in acceleration and to a nonlinear closed loop control of the air path.
For all optimised one-step or two-step PCR assays, the interassay CVs were lower than 10%, demonstrating good reproducibility for all tested kits [ 23].
Of the five one-step kits tested using the Mx3005P cycler, three optimised one-step kits (i.e., Quantitect-1 step, Rotor Gene-1 step, and SSIII Platinium-1 step) revealed good sensitivity with the detection of at least 10 copies/ µL.
When combined with the developed regeneration procedure [1] we show that novel medium compositions and optimised culture steps offer a complete way leading from canes to embryogenic callus initiation and to subsequent plant regeneration.
The two other optimised one-step assays (i.e., Verso-1 step and qScript-1 step) revealed poor sensitivity with a detection of 10 and 10 copies/ µL, respectively.
We found that the optimised one-step PCR assays had a higher detection sensitivity than the optimised two-step assays regardless of the machine used, while no difference was detected in reaction efficiency, R values, and intra- and interreproducibility between the two methods.
In our experience, regardless of the real-time cycler used, one-step qRT-PCR showed better limit of detection at 95% than the optimised two-step assays using pan Lyssavirus primers.
The height of each riser was optimised in Step II using a detailed FE model with the optimised riser positions from Step I.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com