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We additionally generated a full-length HMM profile from the RASCAL optimised alignment with the HMMER package [ 39] to provide a simple way to identify new members of the gene family in the future using a library of HMM profiles.
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The options used here are optimised for alignments where the percent sequence identity is typically 91% [ 25].
Business organisations have been increasingly adopting business process management (BPM) as a part of their core business strategy due to benefits such as improved business information technology (IT) alignment and optimised operational efficiency.
All sequences were manually analysed and assembled with CodonCode Aligner (version 1.5.2).. Multiple sequences were aligned with CLUSTAL W [ 42] and optimised manually with BioEdit Sequence Alignment Editor (version 7.0.1).
Single and concatenated protein sequences were aligned using MUSCLE [ 73] and each alignment was further optimised using GBlocks [ 74].
The two-dimensional structure of the three α-chains and one β-chain were predicted using five prediction programs (discrimination of secondary structure class; nearest neighbour secondary structure, profile network from Heidelberg; self-optimised prediction method from multiple alignment, SSPAL).
The CR data was initially aligned using CLUSTAL X [52] and subsequently both datasets were optimised by visual inspection in a manual alignment editor [53].
To investigate the effect of different query alignments, we first repeated the analysis using query repeat alignments that had been further optimised.
Clustal V was used to create the original alignment that was manually further optimised and redundant sequences were discarded using the edit option of the MUST package.
Accordingly, 'futuring' helps strategically aligning decision-making of multiple actors from multiple sectors at multiple levels by common vision-building (perception-alignment) and goal-setting (goal-alignment), and thus contributes to effective constituency building leading to optimised impact of orchestrated RTDI investments.
Contigs of mitochondrial (mt) genome origin were initially identified using nucleotide BLAST at an E value threshold of 0.001 through alignment of a database containing all contigs from the optimised Velvet assembly (as described above) against the mt genome of the N. lolii standard endophyte (SE) strain as a reference [ 37] (Genbank accession number KF906135).
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CEO of Professional Science Editing for Scientists @ prosciediting.com