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A Box Behnken design was used to optimise the extraction conditions for polysaccharide from Nostoc commune.
The central composite, non-factorial design was used to optimise the extraction conditions, using the Statistica, version 5 software (Statsoft).
Studies were carried out to assess the extraction yield of tomato waste carotenoids in different solvents and solvent mixtures and to optimise the extraction conditions for maximum recovery.
Multivariate analysis was used to optimise the extraction variables (ethanol:water:acetone solvent proportions, times, and acid concentrations) at different levels.
These data allowed us to define new basic parameters for the malaxer design and to optimise the extraction performance, thus ensuring the production of high-quality olive oil.
A simplex-centroid design comprising three solvents (water, ethanol and methanol) was used to optimise the extraction mixture for phenolics and antioxidant activities from defatted soy flour fermented with Monascus purpureus or Aspergillus oryzae.
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The method has been successfully analyse the T and ET in human urine with a detection limit of 1 ng/mL and linear range of 10 500 ng/mL for both compounds by optimising the extraction conditions (type and amount of salts, concentration of analytes, and temperature).
Mussatto et al. (2011) optimised the extraction of antioxidant phenolic compounds from SCG and found that extraction using 60% methanol in a solvent/solid ratio of 40 ml/g SCG, for 90 min, was the most appropriate condition to produce an extract with 16 mg GAE/g SCG of phenolic compounds having high antioxidant activity, i.e. Ferric reducing antioxidant power (FRAP) of 0.10 mM Fe(II)/g).
Central composite design "2n + star" was used in order to optimise the following extraction parameters: number of extractions, composition of the extractant mixture, extraction time and extraction temperature.
Factorial experimental design was carried out to optimise the scCO2 extraction, with 350 bar and 50 °C yielding the selective extraction of higher value components.
To optimise the DNA extraction method for our study, two different DNA-extraction protocols were compared.
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