Exact(1)
Statistically designed experiments were performed to optimise temperature, enzyme to substrate ratio, substrate concentration, agitation, reaction time, pH, buffer concentration and co-solvent addition.
Similar(59)
Incubation, which optimises temperature for chick development, usually begins after the last egg has been laid.
A response surface methodology (RS M approach is applied to optimise the temperature-programme gas-chromatographic separation of 16 organochloride pesticides, including 12 compounds identified as highly toxic chemicals by the Stockholm Convention on Persistent Organic Pollutants.
Extensive finite-element computer simulations were carried out during the design step to optimise the radial temperature gradient up to 1200°C/mm.
Glass transition and crystallisation temperatures and their respective activation energies were determined; extrapolated onset values for these parameters have been determined in order to optimise the sintering temperature for producing amorphous coatings.
To optimise interpretation of temperature measurements and to demonstrate the reliability of the technique to differentiate between infected and healthy livestock requires further IRT data from uninfected animals, kept at different ambient temperatures and under different husbandry conditions.
The PCR conditions were: 95 °C for 10 min followed by 40 cycles of 95 °C for 30 s, optimised primer annealing temperature for 1 min and 72 °C for 1 min.
Hence, we optimised the melting temperature with gradient PCR, using both converted and non-converted DNA and water to minimise false-positives and these controls were included in every amplification step from then on.
In this study, the aim was to optimise the mould surface temperature and the blown bottle's residence time inside the mould for 1 L soft drink PET bottle production based on the final properties using the ECHIP 7 design of experiment (DOE) program.
Biological functions are governed by thermodynamics, and animals regulate their body temperature to optimise cellular performance and to avoid harmful extremes.
Enzymatic extraction of stevioside from Stevia rebaudiana leaves with cellulase, pectinase and hemicellulase, using various parameters, such as concentration of enzyme, incubation time and temperature, was optimised.
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