The individual cassettes carried yeast codon-optimised sequences Ter (from T. denticola), Cct, Adhe2, Bcd and Hbd (from Clostridium Beijerinckii) with a C-terminal Flag tag (two Flag peptide epitopes) and the CYC1 terminator sequences downstream.
A number of phase I clinical trials have established the safety and tolerability of Prostvac-VF [ 65, 66], and a phase II study was used to optimise the sequence of vector administration (one Vaccinia prime followed by three Fowlpox boosts) [ 67].
Given the above constraints and production modes, the product scheduling technique proposed in this paper aims at optimising the sequence of orders in a single machine so that the cost of production (mainly determined by the energy) is minimised.
To optimise the sequencing process, we used 5-base barcodes (#1: 5'-GATCT-3'; #2: 5'-ATCGT-3'; #3: 5'-TGTCT3'; #4: 5'-GTGAT-3') linked to the ligated oligos, in order to be able to pool 3 or 4 individuals per array and to discriminate the sequences obtained from each individual.
Most MRI studies on articular cartilage have tried to optimise pulse sequences that accentuate the contrast-to-noise ratio for cartilage, but less attention has been paid to image resolution.
Optimising drug sequencing in patients with mRCC to extend disease control is a key area of clinical research (Gore and Larkin, 2011; Hudes et al, 2011).
Planning a semi automated assembly line means to allocate the assembly processes on humans and robots and optimise the operating sequence.
All these factors account for and should be considered when attempting to optimise schedules and sequence.
Other PCR reactions failed to optimise, gave unusable sequence or were affected by multiple indel events which prevented analysis.
ESA's Earth observation satellite Envisat was successfully launched on 1 March 2002 by an Ariane-5 launcher, and ESOC immediately took over the task of determining and predicting the orbit using S-band tracking data, and optimising the manoeuvre sequence to bring the spacecraft into an orbit accurately phased with ERS-2.
