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It can be synergistically combined with Sanger sequencing to optimise detection of rare mutations.
HRG was used at 2 ng/ml to optimise detection of synergism between HRG and LL-37.
They optimise detection of relevant autoantibodies while minimising detection of low-titre nonspecific antibodies (known to accumulate with advancing age) and poly-reactive IgM antibodies [ 16, 17].
These settings have been altered, by either different users or by the instrument, to optimise detection of as many peptides as possible for each experiment.
Therefore, procedures to optimise detection of severe infection were partially applied in our patients that did not seem to be perceived as severely ill.
Monthly rescreening has been has been reported in some series [ 18], which may optimise detection of maternal seroconversion and reduce the risk of fetal infection prior to CMV HIG administration, but the feasibility and cost effectiveness of such an approach on a large scale remains to be determined.
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We have developed a spotted genome wide microarray chip with oligonucleotides printed in duplicate in order to minimise undesirable biases, thereby optimising detection of true differential expression.
Services, which optimise detection and management of older patients with mental health problems, need to be developed and evaluated.
In our own studies, the identification of HPV DNA proved to be difficult and required additional amplification of the DNA before PCR and the use of SYBR Green I to optimise detection (Kan et al, 2005).
Meticulous technique is required to optimise detection rate (online appendix 3).
To optimise molecular detection of herpesviruses an internally controlled multiplex Taqman-PCR for the detection of Herpes simplex virus 1 (Herpes Herpesimplexex virus 2 (HSV2) and Varicella-zoster virus (VZV) was developed.
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