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After optimisation, the sensitivity and specificity of the rRT-PCR were established.
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This independent-loop design was versatile since it enabled a separate optimisation of the sensitivity and RF field distributions of the two coils.
The optimisation of the sensitivity in the ICP-MS determination of 83 isotopes, as a function of 21 operative parameters was performed by generating an initial experimental design that was used to define, by principal component analysis, the multi-criteria target function.
This procedure is based on the fact that while the magnitude of either the shaking force or the shaking moment can be bounded through including a set of constraints in the optimisation algorithm, the sensitivities of the other, either those of the shaking force or the shaking moment, can be minimised.
The model response is fitted best to the data with a gradient-based optimisation strategy, requiring the sensitivity analysis of the micromechanical model.
The bound optimisation model and the sensitivity analyses were carried out using Mathematica, V.6.0, and the statistical analyses were performed using SPSS for Windows, V.16 (2002).
The unknown parameters are identified to be the solution to an optimisation problem, which involves using the sensitivity analysis and scatters of the modal identification.
By exploiting the representation of such models as an equivalent optimisation problem, classical results on the sensitivity analysis of non-linear programs may be applied, to produce linear relationships between input data perturbations and model outputs.
However, the sensitivity to optimisation of in situ hybridisation makes it problematic for high throughput analysis.
Our data indicate that the true need in diagnostic testing lies not in further optimisation of sensitivity, but in improving the specificity of the results.
For optimisation and sensitivity testing of the custom array, total RNA was isolated from ex vivo LPS-stimulated mouse peritoneal macrophages with the Aurum total RNA mini kit (BioRad), according to manufacturer's instructions, and RNA yield was measured using a NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA, USA).
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