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5′-DIG and 3′-DIG labelled detection probes specific for mouse miR-133α (Cat. No. 39270-15) and miRCURY LNA microRNA ISH optimisation Kit (FFPE) were purchased from Exiqon, and the experiments were performed according to the manufacturer's instructions.
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Here, we describe the optimisation of the commercially-available LANCE cAMP detection kit (PerkinElmer) to enable detection in cell lysates.
The protocol for chromatin immunoprecipitation was based on the EZ-ChIP kit (Upstate) and manufacturer's procedures, with optimisation for the cell lines and antibodies tested.
Human and viral DNA were extracted using the Qiagen UK kit (Crawley, West Sussex, UK) (QIAamp® DNA Mini Kit) following optimisation of the manufacturer's protocol.
This logic of optimisation has clear limits.
Kit sighed.
As a performance optimisation, AsyncHttpClient pools Netty channels for reuse.
The technical solution it chose was voltage optimisation.
Kit Buchan.
After all, SEO (search engine optimisation) is all about content.
As previously stated, considerable effort goes into search engine optimisation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com