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Our results show that RA3 was most optimally expressed in the C43 DE3 pLysS strain of E. Coli at 37 ℃with a 3 hour growth time and isolated with a NiNTA Agarose column followed with step elution of imidazole.
However, other regulatory mechanisms may cause this RNA to be most optimally expressed at different growth phases.
On the other hand, genes requiring more than one or two pMAPKs, would be optimally expressed only at frequencies at which all the requisite MAPKs are activated simultaneously at the highest levels.
The αGSU, which depends only on pERK1/2 for activation, might be optimally expressed at GnRH frequencies at which only ERK1/2, but not JNK or p38 are highly activated.
As peak Dazl mRNA levels were not observed until the later stage of culture, it is possible that in order for Dazl to be optimally expressed, the induction of other key germ cell determinants would first need to occur.
Conformation modelling revealed the sequence to be optimally expressed by the intact folded protein.
Similar(44)
"Their work is basically trying to advise different vineyardists how they can optimally express their unique terroir". They have identified micro-sites within Popelouchum and advised on which rootstocks and which varieties to plant where, he said.
We sought to optimally express a miRNA simultaneously with a fluorescent marker.
But the essential feature of targets is that they specify the kind, degree or extent of achievement of a goal, which, optimally, is expressed quantitatively.
They may therefore function sub-optimally when expressed in males, which could result in male-biased mortality rates [ 14].
This data is in agreement with our model that PrrF1 and PrrH are transcribed from the same Fur-repressed promoter, and that both are expressed optimally under iron-depleted conditions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com