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The second step involves generating optimal templates of the spikes from a segment containing both noise and multiunit activity.
As proposed by Bohorquez and colleagues [15], [16] optimal templates should be obtained by using jitter stimulation sequences close to steady-state rates.
We assume that among available reference haplotypes, the optimal templates for imputing T are the descendants of the lineage with which T first coalesces.
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Further, after optimizing primers and probes and determining optimal template dilutions for each qPCR target, by running standard curves for all targets on each qPCR plate, one can logistically side-step two common qPCR pit-falls: 1).
The optimal template represents essential geological features of the training image, and its size is much smaller than the template size selected by entropy.
Based on this, we introduce the correlation coefficient matrix to determine an optimal template, and the accuracy of template selection algorithm is also discussed.
To identify the optimal template structure for the loops/junctions, the model screens the pretabulated template library according to the loop size (first) and the sequence (second) matches.
One of the challenging problems in designing templates is to find the optimal template that functions appropriately for the solution of the intended problem.
But contrary to our proposed method, their maximum margin temporal warping (MMTW) method relies on a costly SVM algorithm in order to extract the optimal template for the training dataset.
Optimal template selection, and target-to-template alignment is key to the success of any modeling exercise [40], [41].
The highly sensitive real time PCR (Q-PCR) methods developed for this purpose are usually standardized under optimal template conditions.
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