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In our future work, we are particularly interested in finding more generic and more optimal methodologies to close the gap with respect to the theoretically achievable performance; to this end, we intend to investigate, e.g., machine-learning-based approaches.
However, in order for such clinical applications to be developed, further work is required to determine which are the optimal methodologies to quantify methylation status accurately and reproducibly.
The general lack of clinical responses evoked important questions concerning the optimal methodologies for DC vaccine preparation as well as the design of clinical vaccination protocols.
The quantitative analysis of DNA methylation patterns has the potential to serve as diagnostic and prognostic biomarkers, however, there is currently a lack of consensus regarding the optimal methodologies to quantify methylation status.
Although advances in sequencing technologies have popularized the use of microRNA (miRNA) sequencing (miRNA-seq) for the quantification of miRNA expression, questions remain concerning the optimal methodologies for analysis and utilization of the data.
Recently, simpler alternatives of 'small-intelligent libraries' designed by the program DC-analyzer [ 8] and the '22c trick' [ 9] have been described, which are optimal methodologies to saturate small numbers of codons effectively and efficiently, irrespective of location; although, owing to multiplex PCR primers, these approaches cannot saturate larger numbers of codons (see below).
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The aim of the present article was to give an update on the prevalence of the most important headache types in Europe, to identify gaps in our present knowledge and to analyse some methodological issues in order to choose an optimal methodology for the studies to be performed for the EUROLIGHT project.
An optimal methodology of sensor allocation is then proposed.
First, poor attention to reporting optimal methodology in AR confounds the interpretation and extrapolation of experimental results [5],[5]]–[5]].
The optimal methodology generally is to combine ANN with statistical and/or geostatistical methods in predicting reservoir properties.
However, due to budget limitations, an optimal methodology to estimate the integrity of a structure at minimum cost is required.
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