Results of optimization suggest that the optimal medium composition is (g/L): glycerol (13.92), yeast extract (5.00), phosphates (0.63), CaCO3, (3.0), NaCl, (3.0), MgSO4, (0.5).
The experimental data was analyzed to obtain the regression model and the optimal medium composition was achieved by optimization with UD 3.0 software.
Furthermore, the suitable culture conditions were optimized using single-factor experiments and the optimal medium component was developed using PB design and RSM.
We next sought to optimize the generation of TuJ+ cells and so to develop an optimal medium by testing dropouts of each medium component (Fig. 1a, Additional file 1: Figure S1 and Table S1).
Optimal medium components were determined using fractional factorial design.
In this paper, the optimal medium for higher yield of TL1-1 was first obtained.
An optimal medium to culture Chlorella sp., microalgae capable of storage intracellular lipids was obtained.
With this optimal medium, both the chitinase activity and cell growth were remarkably enhanced.
With the optimal medium, the highest hydroxyl radical inhibition (58.16%) was achieved.
The optimal medium allowed bacteriocin yield to be doubled compared to CM medium.
The optimal medium composition was developed by applying the Plackett Burman experimental design.
