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This level proved to be too low for optimal suppression of hepatic glucose output and optimal glucose uptake (11).
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In in vitro assays, 0.2 mg/mL AA in the incubation media was optimal for glucose uptake in isolated soleus muscle slices.
Insulin-induced microvascular recruitment is important for optimal muscle glucose uptake.
The asymmetry of the interval chosen was introduced to take into account that the sum of the fluxes, in optimal growth conditions, is approximately proportional to glucose uptake (see Additional file 7), making more likely flux decreases than flux increases when glucose uptake is decreased.
Interestingly, the glucose uptake dynamics also differs between optimal and recovery conditions.
Indeed, the optimal solution predicts a metabolic switch, in which glucose uptake rates larger than f1 leads to a linearly increasing lactate production, and therefore a component of the ATP production is now derived from aerobic glycolysis.
The second hypothesis is that glucose uptake profiles for cells grown under optimal conditions differ from the cells grown under heat stress conditions (preconditioning).
In region II, the optimal growth of iAK692 was linearly dependent on both bicarbonate and glucose uptake fluxes.
The results indicate that a change in temperature from optimal to heat stress conditions (30°C to 39°C) produced significant differences in glucose uptake profiles.
Thus, we intend to analyze the following questions: First, if one adjusts for the differences in initial biomass, are the glucose uptake profiles essentially the same or do they significantly differ under optimal, heat stress and recovery conditions?
In the wild-type seen in Figure 6-A, optimal growth can occur across a wide range of ethanol flux values, limited by the maximum glucose uptake rate.
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