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The ingredients contained in mammalian urine are not optimal for cell survival.
However, non-charged surface of PVA-based hydrogels is not optimal for cell adhesion and spreading.
Although degradable and nondegradable polymer matrices offer adequate mechanical stability, they are not optimal for cell adherence and growth.
In terms of an appropriate carrier material, collagen scaffolds with homogeneous pore size of 65 μm were optimal for cell seeding and cultivating.
Thus, the standard ISO 10993-5 procedure is inappropriate for cytotoxicity evaluation of ceramics of high specific surface area because biomaterial extract obtained in this method (ions-depleted medium) is not optimal for cell cultures per se.
At the core of cartilage bioengineering are 3 essential elements: chondrocytes and the application of cell sources capable of undergoing chondrogenic proliferation and differentiation; the application of biological growth factors optimal for cartilage repair; and the utilization of scaffolds optimal for cell and growth factor delivery and maturation.
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Cell proliferation was measured with the Alamar Blue dye reduction method (Biosource International, Camarilo, CA, USA), which is optimal for cells growing in anchorage-independent conditions.
Thicker (30-μm) sections were used to increase the sensitivity for markers of nerves, particularly fine, penetrating IENFs, whereas thinner (15-μm) sections were optimal for cells detected with inflammatory cell markers.
This study was designed to determine the optimal time for cell transplantation after myocardial injury.
CCAST produces an optimal strategy for cell sorting by automating the selection of gating markers, the corresponding gating thresholds and gating sequence; all of these parameters are typically manually defined.
Site-selective detection provides the capability of defining optimal locations for cell capture based on various channel geometries and flow profile.
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